Ali RIŞVANLI

Article | 2022 | Journal of the Hellenic Veterinary Medical Society73 ( 2 )

The immune system of the cow is kept strong to protect the cows from mastitis, which causes economic losses for dairy cattle herds. Cellular immunity, especially, plays an important role as a first-line defense system. In the case of inflammation, cytokines play a decisive role in monitoring this process. In this study, the relationship between somatic cell count (SCC) and cytokine (tumor necrosis factor alpha, interferon gamma, and interleukins TNF-alpha, IFN-gamma,IL-2, -4, -5, and -10, respectively) concentrations in milk were determined. It was decided to support the humoral and cellular aspects of the udder defense system by de . . .termining the T-helper cell (Th1/Th2) cytokine polarization in high SCC milk from cows with signs of mastitis. Milk samples from 180 cows were divided into five groups according to the somatic cell count level (< 150 x 10(3), 151 x 10(3) 400 x 10(3), 401 x 10(3)-600x10(3), 601 x 10(3)-1,000 x 10(3), and > 1,000x10(3)cells/mL). It was understood that the Th1 direction was determined before mastitis was formed, that is when SCC was low (< 150 x 10(3)). As a result. it was concluded that theTh1 polarization should be supported to protect cows from mastitis More less

Ayday CUNUŞOVA

Article | 2022 | Journal of the Hellenic Veterinary Medical Society73 ( 4 )

This study aimed to compare three ways of FSH administration [single-dose subcutaneous (sc) using an adjuvant, single-dose epidural without an adjuvant and the conventional intramuscular (i.m) repeated decreasing doses] on blood FSH levels and superovulation response. Holstein cows (n:18) were divided into three groups for the superovulation procedure (n6). The animals in group (1) received intramuscular (im) decreasing doses of FSH (Stimufol, 500 mu g porcine FSH and 100 mu g porcine LH, Ulg FMV, Liege, Belgium) at 12-h intervals for 4 d. The animals in group (2) received FSH (500 mu g) into the epidural space (e). The animals in g . . .roup (3) received a single subcutaneous (SC) dose of FSH (500 mu g) added in 10 mL of Montanide ISA 206 adjuvant. CL count in the ovary was measured by ultrasonography and superovulation was determined based on the presence of >4 CL. For FSH measurement, blood samples were collected from the V. jugularis of all the animals. Serum FSH (pFSH) concentration was determined by ELISA. After synchronisation and superovulation procedures, all animals had estrus (100). Superovulation response (> 4 CL) was detected in six animals in group 1 (im), one animal in group 2 (e), and four animals in group 3 (sc). Superovulation responses in e group were lower than those in im group (P0.05). Serum FSH concentrations were similar between groups for all sampling times (P>0.05). Keyword: epidural; FSH; holstein cow; montanide; superovulation respons More less

Ali RIŞVANLI

Article | 2023 | Journal of the Hellenic Veterinary Medical Society74 ( 2 )

The regulation pattern of important enzymes in placental steroidogenesis and prostaglandin production in ewes with pregnancy toxemia is reviewed. The alterations of gene expressions after the administration of aglepristone (AG) and dexamethasone (DEX) are also discussed. Four healthy (CG) and 22 ewes with experimental pregnancy toxemia were included in the study. Ewes with pregnancy toxemia of group AG (n9) and group DEX (n9) were injected twice with 10 mg/kg of aglepristone and once with 5 ml dexamethasone respectively to induce parturition on 141 & PLUSMN;1,3 day of gestation; whereas healthy control [Group CG (n4)] and pregnancy . . .toxemia [Group PT (n4)] group received no treatment for parturition induction. Placentomes were immediately collected right after the expulsion of the last lamb. mRNA extraction from total placentome capsule, cotyledon and caruncle was carried out and Real-Time PCR was performed. Serum samples were collected from ewes and cortisol, PGFM, PGE2, estrone sulfate and progesterone concentrations were measured after treatments until parturition. The lowest mRNA expressions of steroidogenic enzymes were detected in group PT. Interestingly expression pattern of steroidogenic enzymes in group AG was similar to group PT. No difference was found in mRNA expressions of 3 & beta;HSD and CYP19 among groups. Between groups, AG-DEX the mRNA expressions in the caruncle of PTGS2/COX2 and PGFS were statistically different respectively ( More less

İsmail ŞEN

Article | 2022 | Journal of the Hellenic Veterinary Medical Society73 ( 2 )

This study aimed to detect changes in clinical parameters, biochemical and serum cytokine levels in calves with naturally occurring sepsis. In the study, 34 calves diagnosed with sepsis according to clinical and laboratory results and 7 calves deemed to be healthy according to clinical and laboratory results were used. The blood sampling was performed by jugular venapuncture for hematological and biochemical analysis and the determination of cytokine levels. Significant changes were observed in the clinical parameters of calves with sepsis. Serum concentrations of IL-1 beta, IL-6, IL-10, TNF-alpha, and INF-gamma were significantly i . . .ncreased ( More less

Hasan GÜZELBEKTEŞ | İsmail ŞEN

Article | 2018 | Journal of the Hellenic Veterinary Medical Society69 ( 4 )

In this study, it was aimed to determine of diagnostic importance of blood lipid levels in neonatal calves with sepsis. The study was carried out on a total of 70 calves, 60 with sepsis and 10 healthy calves. The calves with sepsis were included in the study, according to clinical and hematological findings. The blood samples were taken from the V. jugularis for hematological, lipid profile and biochemical analyzes after the routine clinical examinations of the calves. There were significantly (P < 0.05) decrease in body temperature, increase in respiration rate and capillary refill time in the calves with sepsis compared to control . . . group. The levels of blood urea nitrogen, creatinine concentrations of calves with sepsis were significantly higher (P < 0.05), however, levels of total cholesterol, HDL and LDL concentrations were significantly lower (P < 0.05) than control group. In addition, blood triglyceride and VLDL concentrations of calves with sepsis were higher than control group, however there was no statistical difference. In conclusion, serum total cholesterol, HDL and LDL in neonatal calves with sepsis could be used in evaluation of the sepsis in calves More less